Citation

Tanaka, M., Song, G.-J., Matsuno, R., and T. Kamikubo, Evaluation of effectiveness of pretreating rice straw with n-butylamine for improvement of sugar yield. Applied Microbiology and Biotechnology 22: 19-25, 1985.

Abstract

Summary. n-Butylamine (n-BA) pretreatment extracted a part of the holocellulose (cellulose and hemicellulose) in rice straw into the liquid phase, as a mixture of oligosaccharides and a small amount of monosaccharides. However, there was loss of sugars resulting mainly from decomposition of monomers by a long exposure to a high n-BA concentration. Approximately 70% of the total sugars in the pretreatment solution was converted enzymatically into reducing sugars (mainly monomers). On the other hand, more than 90% of holocellulose in the residual rice straw after the optimal pretreatment was solubilized enzymatically in 120h of reaction time with 10 w/v8/0 substrate and 6 mg protein/ml cellulase. The main action of n-BA on rice straw was delignification, and highly crystalline cellulose was not swollen. Thus the enhancement of the enzymatic solubilization rate of rice straw appeared to be due to the increase of surface area accessible to the enzyme be delignification. It was demonstrated by the relationship of the vapor-liquid equilibrium of the n-BA/water system that the n-BA used was recovered easily. The loss of n-BA, at most, was 30% in the case of the weight ratio of n-BA to rice straw of 0.1

Country, State, District, etc.

Japan

Language

English

Material

rice straw

Use category

ethanol production, single cell protein