Citation

Ram, M. S. and G. Seenayya, Production of ethanol from straw and bamboo pulp by primary isolates of Clostridium thermocellum. World Journal of Microbiology and Biotechnology 7: 372-378, 1991.

Abstract

Clostridium thermocellum strains S58 and GS1 grew poorly on crude biopolymere but fermented them easily after alkali treatment. With 1% alkali-extracted rice straw (AERS) and delignified bamboo pulp (DBP), he ethanol-to-substrate (E/S) ratios were almost the same as those obtained when using fuller paper, increasing the substrate concentrations decreased the percentage substrate degraded and the E/S ratio and concomitantly increased the amount of reducing sugars accumulated. A maximum amount of 8.6g ethanol/l was produced by strain S58 out o f37.5 g DBP degraded. Strain G51 accumulated reducing sugars of substrate concentrations >50g/l, thereby accounting for about 70% of AERS degraded. This strain produced cellulase on both cellulose and cellulase. Both the strains grew in the presence of 1.5% (v/v) ethanol. Strain S58 fermented starch, but the ethanol yield was low compared to that from cellulose. About 75% of starch degraded accumulated at reducing sugars at a substrate concentration of 40g/l. Tehinhibitroy effects of ethanol (2 to 4%) were less drastic when growing cultures were challenged than when they were formed in situ. The effect of ethanol depended upon the phase of the culture.

Country, State, District, etc.

India

Language

English

Material

rice straw, bamboo pulp

Use category

ethanol production