Citation

Arai, M., Ooi, T., Hayashi, H., and S. Murao, Conversion of rice straw to ethanol by simultaneous saccharification and fermentation. Hakkokogaku 63: 427-431, 1985.

Abstract

When various concentrations of alkali-treated rice straw were hydrolyzed by a mixture of cellulases Acurelase form Aspargilus aculeatus and Meicelase from Trichederma virida, 5-10% rice straw was well hydrolyzed. On the contrary 20-25% rice straw was hardly hydrolyzed, because of difficulties of stirring and product inhibition. To solve this barrier, simultaneous saccharification and fermentation of rice straw was examined. When 25% rice straw was fermented by a mixed cellulase, xylose isomerase and Saccharomyces fermentati IAM 4771, 25% rice straw was hydrolyzed almost completely but ethanol yield was relatively low. This phenomenon seemed to be due to tolerance of the yeast to a high concentration of ethanol and ß-1.3-glucanase contaminating the cellulase preparation. From screening for a suitable strain for simultaneous saccharification and fermentation, Saccharomyces cerevisiea AJ 4003 were selected. When 25% rice straw was fermented by a mixed cellulase, xylose isomerase and Saccharomyces cerevisiae AJ 4005, 11.1-11.5% of ethanol was obtained. The cellulase concentration required was 0.5%. The ethanol yield was 92-95% of the theoretical value.

Country, State, District, etc.

Japan

Language

Japanese (English title and abstract)

Material

rice straw

Use category

ethanol production