Citation

Peiris, P. S. and I. Silva, Hydrolysis of rice straw to fermentable sugars by Trichoderma enzymes. MIRCEN Journal 3: 57-65, 1987.

Abstract

A Trichoderma sp. (IMB-Tr) isolated from rice straw possessed cellulolytic and xylanolytic activity, comparable to those produced by Trichoderma reesei QM 9414 ( a proven cellulolytic fungus), IMB-Tr produced 2.9 and1.9 times, respectively, greater ?-glucosidase activity compared to T. reesei when grown on microcrystalline cellulose and rice straw. Percentage enzymatic hydrolysis increased with increase in the sodium hydroxide concentration used in the pretreatment of rice straw and with the increase of enzyme concentration used in the hydrolysis. The extracellular enzyme fraction of T. reesei possessed greater hydrolytic power than that of IMB-Tr. However, when a combined enzyme preparation from the two organisms was used, an appreciable degree of synergism was observed; an increase in reducing sugars up to 39% was seen. The reducing sugar produced by enzymatic hydrolysis was mainly glucose, xylose and cellobiose. Fermentation of a 4.8% (w/v) sugar hydrolysate (produced by the enzymic hydrolysis of rice straw) by Saccharomyces cerevisiae produced 10.7 g/l of ethanol compared to 18.8 g/l produced by the fermentation of 4.8% (w/v) pure glucose.

Country, State, District, etc.

Sri Lanka

Language

English

Material

rice straw

Use category

energy production, ethanol production, chemical production, single cell protein